Antibody responses to individual proteins of SARS coronavirus and their neutralization activities.
Identifieur interne : 004928 ( Main/Exploration ); précédent : 004927; suivant : 004929Antibody responses to individual proteins of SARS coronavirus and their neutralization activities.
Auteurs : Maofeng Qiu [République populaire de Chine] ; Yuling Shi ; Zhaobiao Guo ; Zeliang Chen ; Rongqiao He ; Runsheng Chen ; Dongsheng Zhou ; Erhei Dai ; Xiaoyi Wang ; Bingyin Si ; Yajun Song ; Jingxiang Li ; Ling Yang ; Jin Wang ; Hongxia Wang ; Xin Pang ; Junhui Zhai ; Zongmin Du ; Ying Liu ; Yong Zhang ; Linhai Li ; Jian Wang ; Bing Sun ; Ruifu YangSource :
- Microbes and infection [ 1286-4579 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : Anticorps antiviraux.
- immunologie : Anticorps antiviraux, Immunoglobuline G, Protéines virales, Virus du SRAS.
- Animaux, Humains, Lapins, Tests de neutralisation.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Antibodies, Viral.
- chemical , immunology : Antibodies, Viral, Immunoglobulin G, Viral Proteins.
- immunology : SARS Virus.
- Animals, Humans, Neutralization Tests, Rabbits.
Abstract
A novel coronavirus, the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), was identified as the causative agent of SARS. The profile of specific antibodies to individual proteins of the virus is critical to the development of vaccine and diagnostic tools. In this study, 13 recombinant proteins associated with four structural proteins (S, E, M and N) and five putative uncharacterized proteins (3a, 3b, 6, 7a and 9b) of the SARS-CoV were prepared and used for screening and monitoring their specific IgG antibodies in SARS patient sera by protein microarray. Antibodies to proteins S, 3a, N and 9b were detected in the sera from convalescent-phase SARS patients, whereas those to proteins E, M, 3b, 6 and 7a were undetected. In the detectable specific antibodies, anti-S and anti-N were dominant and could persist in the sera of SARS patients until week 30. Among the rabbit antisera to recombinant proteins S3, N, 3a and 9b, only anti-S3 serum showed significant neutralizing activity to the SARS-CoV infection in Vero E6 cells. The results suggest (1) that anti-S and anti-N antibodies are diagnostic markers and in particular that S3 is immunogenic and therefore is a good candidate as a subunit vaccine antigen; and (2) that, from a virus structure viewpoint, the presence in some human sera of antibodies reacting with two recombinant polypeptides, 3a and 9b, supports the hypothesis that they are synthesized during the virus cycle.
DOI: 10.1016/j.micinf.2005.02.006
PubMed: 15878679
Affiliations:
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uniqKey="Guo Z" first="Zhaobiao" last="Guo">Zhaobiao Guo</name></author><author><name sortKey="Chen, Zeliang" sort="Chen, Zeliang" uniqKey="Chen Z" first="Zeliang" last="Chen">Zeliang Chen</name></author><author><name sortKey="He, Rongqiao" sort="He, Rongqiao" uniqKey="He R" first="Rongqiao" last="He">Rongqiao He</name></author><author><name sortKey="Chen, Runsheng" sort="Chen, Runsheng" uniqKey="Chen R" first="Runsheng" last="Chen">Runsheng Chen</name></author><author><name sortKey="Zhou, Dongsheng" sort="Zhou, Dongsheng" uniqKey="Zhou D" first="Dongsheng" last="Zhou">Dongsheng Zhou</name></author><author><name sortKey="Dai, Erhei" sort="Dai, Erhei" uniqKey="Dai E" first="Erhei" last="Dai">Erhei Dai</name></author><author><name sortKey="Wang, Xiaoyi" sort="Wang, Xiaoyi" uniqKey="Wang X" first="Xiaoyi" last="Wang">Xiaoyi Wang</name></author><author><name sortKey="Si, Bingyin" sort="Si, Bingyin" uniqKey="Si B" first="Bingyin" last="Si">Bingyin Si</name></author><author><name 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sortKey="Liu, Ying" sort="Liu, Ying" uniqKey="Liu Y" first="Ying" last="Liu">Ying Liu</name></author><author><name sortKey="Zhang, Yong" sort="Zhang, Yong" uniqKey="Zhang Y" first="Yong" last="Zhang">Yong Zhang</name></author><author><name sortKey="Li, Linhai" sort="Li, Linhai" uniqKey="Li L" first="Linhai" last="Li">Linhai Li</name></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name></author><author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name></author><author><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15878679</idno><idno type="pmid">15878679</idno><idno type="doi">10.1016/j.micinf.2005.02.006</idno><idno type="wicri:Area/PubMed/Corpus">002754</idno><idno 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uniqKey="Qiu M" first="Maofeng" last="Qiu">Maofeng Qiu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Shi, Yuling" sort="Shi, Yuling" uniqKey="Shi Y" first="Yuling" last="Shi">Yuling Shi</name></author><author><name sortKey="Guo, Zhaobiao" sort="Guo, Zhaobiao" uniqKey="Guo Z" first="Zhaobiao" last="Guo">Zhaobiao Guo</name></author><author><name sortKey="Chen, Zeliang" sort="Chen, Zeliang" uniqKey="Chen Z" first="Zeliang" last="Chen">Zeliang Chen</name></author><author><name sortKey="He, Rongqiao" sort="He, Rongqiao" uniqKey="He R" first="Rongqiao" last="He">Rongqiao He</name></author><author><name sortKey="Chen, Runsheng" sort="Chen, Runsheng" uniqKey="Chen R" first="Runsheng" last="Chen">Runsheng Chen</name></author><author><name sortKey="Zhou, Dongsheng" sort="Zhou, Dongsheng" uniqKey="Zhou D" first="Dongsheng" last="Zhou">Dongsheng Zhou</name></author><author><name sortKey="Dai, Erhei" sort="Dai, Erhei" uniqKey="Dai E" first="Erhei" last="Dai">Erhei Dai</name></author><author><name sortKey="Wang, Xiaoyi" sort="Wang, Xiaoyi" uniqKey="Wang X" first="Xiaoyi" last="Wang">Xiaoyi Wang</name></author><author><name sortKey="Si, Bingyin" sort="Si, Bingyin" uniqKey="Si B" first="Bingyin" last="Si">Bingyin Si</name></author><author><name sortKey="Song, Yajun" sort="Song, Yajun" uniqKey="Song Y" first="Yajun" last="Song">Yajun Song</name></author><author><name sortKey="Li, Jingxiang" sort="Li, Jingxiang" uniqKey="Li J" first="Jingxiang" last="Li">Jingxiang Li</name></author><author><name sortKey="Yang, Ling" sort="Yang, Ling" uniqKey="Yang L" first="Ling" last="Yang">Ling Yang</name></author><author><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name></author><author><name sortKey="Wang, Hongxia" sort="Wang, Hongxia" uniqKey="Wang H" first="Hongxia" last="Wang">Hongxia Wang</name></author><author><name sortKey="Pang, Xin" sort="Pang, Xin" uniqKey="Pang X" first="Xin" last="Pang">Xin Pang</name></author><author><name sortKey="Zhai, Junhui" sort="Zhai, Junhui" uniqKey="Zhai J" first="Junhui" last="Zhai">Junhui Zhai</name></author><author><name sortKey="Du, Zongmin" sort="Du, Zongmin" uniqKey="Du Z" first="Zongmin" last="Du">Zongmin Du</name></author><author><name sortKey="Liu, Ying" sort="Liu, Ying" uniqKey="Liu Y" first="Ying" last="Liu">Ying Liu</name></author><author><name sortKey="Zhang, Yong" sort="Zhang, Yong" uniqKey="Zhang Y" first="Yong" last="Zhang">Yong Zhang</name></author><author><name sortKey="Li, Linhai" sort="Li, Linhai" uniqKey="Li L" first="Linhai" last="Li">Linhai Li</name></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name></author><author><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name></author><author><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name></author></analytic><series><title level="j">Microbes and infection</title><idno type="ISSN">1286-4579</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (biosynthesis)</term><term>Antibodies, Viral (immunology)</term><term>Humans</term><term>Immunoglobulin G (immunology)</term><term>Neutralization Tests</term><term>Rabbits</term><term>SARS Virus (immunology)</term><term>Viral Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Anticorps antiviraux (immunologie)</term><term>Humains</term><term>Immunoglobuline G (immunologie)</term><term>Lapins</term><term>Protéines virales (immunologie)</term><term>Tests de neutralisation</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Neutralization Tests</term><term>Rabbits</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Lapins</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A novel coronavirus, the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), was identified as the causative agent of SARS. The profile of specific antibodies to individual proteins of the virus is critical to the development of vaccine and diagnostic tools. In this study, 13 recombinant proteins associated with four structural proteins (S, E, M and N) and five putative uncharacterized proteins (3a, 3b, 6, 7a and 9b) of the SARS-CoV were prepared and used for screening and monitoring their specific IgG antibodies in SARS patient sera by protein microarray. Antibodies to proteins S, 3a, N and 9b were detected in the sera from convalescent-phase SARS patients, whereas those to proteins E, M, 3b, 6 and 7a were undetected. In the detectable specific antibodies, anti-S and anti-N were dominant and could persist in the sera of SARS patients until week 30. Among the rabbit antisera to recombinant proteins S3, N, 3a and 9b, only anti-S3 serum showed significant neutralizing activity to the SARS-CoV infection in Vero E6 cells. The results suggest (1) that anti-S and anti-N antibodies are diagnostic markers and in particular that S3 is immunogenic and therefore is a good candidate as a subunit vaccine antigen; and (2) that, from a virus structure viewpoint, the presence in some human sera of antibodies reacting with two recombinant polypeptides, 3a and 9b, supports the hypothesis that they are synthesized during the virus cycle.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Chen, Runsheng" sort="Chen, Runsheng" uniqKey="Chen R" first="Runsheng" last="Chen">Runsheng Chen</name><name sortKey="Chen, Zeliang" sort="Chen, Zeliang" uniqKey="Chen Z" first="Zeliang" last="Chen">Zeliang Chen</name><name sortKey="Dai, Erhei" sort="Dai, Erhei" uniqKey="Dai E" first="Erhei" last="Dai">Erhei Dai</name><name sortKey="Du, Zongmin" sort="Du, Zongmin" uniqKey="Du Z" first="Zongmin" last="Du">Zongmin Du</name><name sortKey="Guo, Zhaobiao" sort="Guo, Zhaobiao" uniqKey="Guo Z" first="Zhaobiao" last="Guo">Zhaobiao Guo</name><name sortKey="He, Rongqiao" sort="He, Rongqiao" uniqKey="He R" first="Rongqiao" last="He">Rongqiao He</name><name sortKey="Li, Jingxiang" sort="Li, Jingxiang" uniqKey="Li J" first="Jingxiang" last="Li">Jingxiang Li</name><name sortKey="Li, Linhai" sort="Li, Linhai" uniqKey="Li L" first="Linhai" last="Li">Linhai Li</name><name sortKey="Liu, Ying" sort="Liu, Ying" uniqKey="Liu Y" first="Ying" last="Liu">Ying Liu</name><name sortKey="Pang, Xin" sort="Pang, Xin" uniqKey="Pang X" first="Xin" last="Pang">Xin Pang</name><name sortKey="Shi, Yuling" sort="Shi, Yuling" uniqKey="Shi Y" first="Yuling" last="Shi">Yuling Shi</name><name sortKey="Si, Bingyin" sort="Si, Bingyin" uniqKey="Si B" first="Bingyin" last="Si">Bingyin Si</name><name sortKey="Song, Yajun" sort="Song, Yajun" uniqKey="Song Y" first="Yajun" last="Song">Yajun Song</name><name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name><name sortKey="Wang, Hongxia" sort="Wang, Hongxia" uniqKey="Wang H" first="Hongxia" last="Wang">Hongxia Wang</name><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name><name sortKey="Wang, Xiaoyi" sort="Wang, Xiaoyi" uniqKey="Wang X" first="Xiaoyi" last="Wang">Xiaoyi Wang</name><name sortKey="Yang, Ling" sort="Yang, Ling" uniqKey="Yang L" first="Ling" last="Yang">Ling Yang</name><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name><name sortKey="Zhai, Junhui" sort="Zhai, Junhui" uniqKey="Zhai J" first="Junhui" last="Zhai">Junhui Zhai</name><name sortKey="Zhang, Yong" sort="Zhang, Yong" uniqKey="Zhang Y" first="Yong" last="Zhang">Yong Zhang</name><name sortKey="Zhou, Dongsheng" sort="Zhou, Dongsheng" uniqKey="Zhou D" first="Dongsheng" last="Zhou">Dongsheng Zhou</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Qiu, Maofeng" sort="Qiu, Maofeng" uniqKey="Qiu M" first="Maofeng" last="Qiu">Maofeng Qiu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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